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Bd facsdiva software user

Bd Facsdiva

For In Vitro Diagnostic Use bdbiosciences.com Part No. 640749 Rev. A May 2006 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax (408) 954-2347 BDFACSDivaSoftwareReferenceManual Asia Pacific Tel (65) 6-861-0633 Fax (65) 6-860-1590 Europe Tel (32) 53-720211 Fax (32) 53-720452 Brazil Tel (55) 11-5185-9995 Fax (55) 11-5185-9895 Japan Nippon Becton Dickinson Company, Ltd. Tel 0120-8555-90 Canada Tel (888) 259-0187 (905) 542-8028 Fax (905) 542-9391 canada@bd.com Mexico Tel (52) 55 5999 8296 Fax (52) 55 5999 8288

BD FACSDiva Software 6.0 Reference Manual. BD Biosciences does not make any warranty with respect to the workstation remaining virus free after installation.

Bd Facsdiva Software User's Guide

  • Page 2 and 3: © 2006, Becton, Dickinson and Comp
  • Page 5 and 6: Contents About This Manual xi Conve
  • Page 7 and 8: Keywords . . . . . . . . . . . . .
  • Page 9 and 10: Statistics . . . . . . . . . . . .
  • Page 11 and 12: About This Manual This manual descr
  • Page 13 and 14: Using Microsoft Windows � Tip To
  • Page 15 and 16: 1 Software Installation and Setup T
  • Page 17 and 18: Adobe® Acrobat® Reader for viewin
  • Page 19 and 20: Compatibility BD FACSDiva software
  • Page 21 and 22: Uninstalling the Current Software T
  • Page 23 and 24: � Tip BD Biosciences recommends t
  • Page 25 and 26: 8 When prompted, select a database
  • Page 27 and 28: 11 Click Finish to complete the ins
  • Page 29 and 30: Starting the Software NOTICE If you
  • Page 31 and 32: Administrative Options If you have
  • Page 33 and 34: The following dialog box appears wh
  • Page 35 and 36: Tracking User Logins BD FACSDiva so
  • Page 37 and 38: 4 Click Import. 5 Select the file c
  • Page 39 and 40: Quitting the Software Do one of the
  • Page 41 and 42: 2 BD FACSDiva Workspace This chapte
  • Page 43 and 44: Workspace Toolbar save view/hide bu
  • Page 45 and 46: To view or hide workspace windows,
  • Page 47 and 48: ascending or descending order. Fold
  • Page 49 and 50: To add… Folders Experiments or ex
  • Page 51 and 52: Using the Current Tube Pointer When
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    Figure 2-8 Example organization of

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    To create an experiment based on a

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    Note that experiment names cannot c

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    3 (Optional) Enter Study Details wh

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    Making Experiments Private or Share

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    Select the Append to currently show

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    Use the following methods to select

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    Exporting a Specimen as a Panel A p

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    specimen based on a panel, each typ

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    Figure 2-13 Tube tab before (left)

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    Use the Keywords tab to view or edi

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    Analysis Objects An Analysis icon i

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    Copying Analyses � Tip When you c

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    Figure 2-14 Creating custom keyword

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    All defined values appear in a drop

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    Start acquisition on pointer change

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    Plot Preferences By default, plots

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    Statistics Preferences Statistics p

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    3 Instrument and Acquisition Contro

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    Instrument Configuration Flow cytom

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    5 To add parameters, click Add; cho

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    The parameter names you enter are t

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    Printing Configurations To print th

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    icon appears at the top of the work

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    area scaling might need a different

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    Instrument Status Report The instru

    31 rows  The software supports audio input from all sources using default application. Microphone free download. Sierra or Another OS on Your Mac Best Graphic Design Software the Mac Stay Safe with. Playback from microphone to speaker. Microphone playback program the best free software for your. This playback feature is a very important or must-have for some applications like Karaoke software or sound mixing programs. Without this feature, you have to spend a lot of money and time to setup a big system with an extra mixer. This program pass the input from microphone through your speakers directly in background to emulate. Jun 21, 2013  Microphone audio playback issue Hey, Mic is picking up some weird noises and sound level is spiking all the time in recording devices when mic is not being used and it only spikes when mic is plugged in, seems like something is being played back on mic when nothing is going on. These voice changer software offer various features, like: change voice (recorded or real time), lets you voice chat with changed voice, can change pitch as well as speed of the saved audio file, can change your voice from male to female, female to male, robot, kid etc., can save the changed voice in various popular audio formats, and more.

  • Page 105 and 106:

    To export the report, choose File >

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    Stopping Gate Events—number of re

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    � Tip You can also set Events to

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    The following examples show how the

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    Adjusting Instrument Settings To ed

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    � Tip Select multiple contiguous

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    Using the Threshold Tab Use the Thr

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    Copying Spectral Overlap Values If

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    Creating Specimen- or Tube-Specific

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    If you make the same changes to tub

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    Exporting Instrument Settings You c

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    When the emission of one fluorochro

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    Leave the Include separate unstaine

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    Figure 3-9 Keeping a generic contro

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    If the compensation calculation is

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    If the selected setup is linked to

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    When the Use global instrument sett

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    If parameters do not match, a messa

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    Figure 3-12 Choosing a control labe

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    2 Create a snap-to gate around the

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    Figure 3-15 Adjusting for FITC spec

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    � Tip Use the Ctrl key and the ar

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    4 Tools for Data Analysis During an

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    To delete a worksheet, click the wo

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    not be displayed in all plots. Any

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    Worksheet Buttons Snap-To Gate butt

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    Editing Worksheets Worksheets can d

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    Copying Worksheet Elements Individu

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    Aligning and Resizing Worksheet Ele

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    Plots Multiparameter data events ca

  • Page 165 and 166:

    Creating Gated Plots After gated po

  • Page 167 and 168:

    Resizing Plots To enlarge a plot, s

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    Choosing Populations to Display Rig

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    Select the Grid checkbox to show gr

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    Editing Plot Titles and Axes Labels

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    percentage value entered. For examp

  • Page 177 and 178:

    of events between each level contai

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    percentage of bins at each end of t

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    Working with the Biexponential Edit

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    NOTICE Manual scaling values apply

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    Disabling Biexponential Display In

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    oundary past the axis to capture al

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    Examples of each type of quadrant g

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    NOTICE Snap-to gates do not work we

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    The following figure shows how adju

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    Editing Gates Any gate can be moved

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    Population Hierarchy All gates and

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    display statistics for a single pop

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    In either case, the new population

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    Statistics coloring of their parent

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    Editing Header Information Use the

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    Editing Parameter Statistics Use th

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    Mean—average linear value for eve

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    Figure 4-21 Exporting statistics 5

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    Select the Statistics checkbox to e

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    The following topics are covered in

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    For optimal performance, follow the

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    Verifying Database Size The databas

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    Exporting and Importing FCS Files D

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    Select the file format: FCS 2.0 (10

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    Follow these steps to import an FCS

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    If an imported tube does not contai

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    3 Make appropriate selections in th Ykr-f/06.

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    Using the Data Manager Utility BD F

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    1 Launch Data Manager. See Using th

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    Restoring a Database Use the Restor

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    6 Troubleshooting The tips in this

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    Electronics Troubleshooting Observa

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    General Software Troubleshooting (c

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    General Software Troubleshooting (c

  • Page 245 and 246:

    Instrument Setup Troubleshooting (c

  • Page 247 and 248:

    Data Manager Troubleshooting Observ

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    Printing Troubleshooting Not all pr

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    Printing 1 In BD FACSDiva software,

  • Page 253 and 254:

    Appendix A Menus and Keyboard Short

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    Shortcut Menus Right-click the indi

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    Objective Key Combination Condition

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    This chapter gives a brief overview

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    Threshold Because BD FACSDiva data

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    Because aborted events are excluded

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    Glossary acquisition tube Tube sele

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    histogram Graphical representation

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    A aborts, electronic about 262 coun

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    chain icon in Browser 136 in plots

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    deleting arrows 158 data 220 experi

  • Page 275 and 276:

    files compatibility 19 installation

  • Page 277 and 278:

    instrument settings about 74, 112 a

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    P pages, worksheet 156 panel templa

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    setup about 134, 136 adding paramet

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    time acquisition 107 stopping 109 T